Technical :

Four real-time PCR kits allow specific detection of SARS-CoV2 virus RNA. The PCR technique, which uses different fluorescent probes and specific primers, allows the amplification of specific genes found in the genome of the SARS-CoV2 RNA virus. The RNAs extracted from the different samples (nasopharyngeal swab, nasopharyngeal aspiration, expectoration, BAL, bronchial aspiration or saliva) are subjected to this test in order to detect SARS-CoV2 virus carriers.

Our kits are among the list of kits allowed in France. They are present on the COVID-19 Platform (sante.gouv.fr)

Also the ADNucleis RNA extraction kits for extraction of various samples recommended for SARS-CoV2 virus detection are available here.

Pre-analytical conditions :

Sample Types: Nasopharyngeal swab, nasopharyngeal aspiration, sputum, BAL, bronchial aspiration, saliva (only in symptomatic patients if nasopharyngeal swabs are not acheivable)

Storage temperature during transport: 4 ° C

PRODUCTS :

  • Direct SARS-CoV-2_1-step RTPCR : Real time PCR kit allow rapid SARS-CoV2 detection , pathogen of the COVID-19 pandemic..
    • Extraction-free kit, it offers undeniable advantages to use:
      • PCR analysis time is devided by 2, since the sample extraction step is reduced to a simple lysis of 5 min. versus 1h30.
      • Plastic consumables are reduced 4 times: use of only 2 tips/sample versus 8.
      • Easier handling, less necessary training and reduced operator risk, as well as reduced use of automates.
    • Ready-to-use Kit: A Master mix tube containing the Taq, the specific primers and probes of each target, a tube containing the RT and a positive control tube.
    • Targets: N and RdRp genes of SARS-Cov2, and human endogenous gene beta-actine used as internal control for extraction and amplification.
    • Contains a triplex PCR: Gene N in ROX, Rdrp1 in Cy5 and beta-actin in HEX.
    • Results in 2 h
    • Packaging: Available in 48 and 96 reactions
    • Transport and storage: International delivery in positive cold and must be stored at -20 ° C upon receipt
    • CE marked kit and validated by ANSM
  • SARS-CoV-2_E_RN: Real-time PCR kit for the detection of SARS-CoV2, pathogen of the COVID-19 pandemic.
    • Ready-to-use kit: One tube of Master mix containing Taq, primers and probes specific for each target, a tube containing RT and a positive control tube.
    • Targets: A sequence in the N gene, a sequence in the Rdrp gene of the SARS-Cov2 virus and a sequence in the beta-actin gene serving as internal control of extraction and amplification.
    • Contains a triplex PCR: N gene in ROX, Rdrp1 in Cy5 and beta-actin in HEX.
    • Results in 2 hours
    • Packaging: Available in tube of 96 reactions
    • Transport and storage: International delivery in positive cold and must be stored at -20 ° C upon receipt
    • CE marked kit and validated by ANSM
  • SARS-CoV-2_U_RN: Real-time PCR kit for the detection of SARS-CoV2, pathogen of the COVID-19 pandemic.
    • Ready-to-use kit: A tube of Master mix containing RT and Taq enzymes, primers and probes specific for each target and a positive control tube.
    • Targets: A sequence in the N gene, a sequence in the Rdrp gene of the SARS-Cov2 virus and a sequence in the beta-actin gene serving as an internal control of extraction and amplification.
    • Contains a triplex PCR: N gene in ROX, Rdrp1 in Cy5 and beta-actin in HEX.
    • Results in 2 hours
    • Packaging: Available in tube of 96 reactions
    • Transport and storage: Delivery to France and Europe in dry ice and must be stored at -20 ° C upon receipt
    • CE marked kit and validated by ANSM
ReferencesDirect SARS-CoV-2_1-step SARS-CoV-2_E_RNSARS-CoV-2_U_RN
Process PCR One stepOne stepOne step
Target genesN (FAM)
RdRp (Cy5)
N (FAM)
Rdrp (Cy5)  
N (FAM)
Rdrp (Cy5)
Internal controlBeta-actin (HEX)Beta-actin (HEX)Beta-actin (HEX)
Sensitivity
(copy/PCR)
N (40)
RdRp (5)
N (10)
RdRp (5)
N (20.1)
RdRp (5)
Nbr of wells111
ValidationANSMANSMANSM
CertificationCECECE
UseTo reconstituteTo reconstituteReady-to-use
Packaging48 or 96 reactions48 or 96 reactions48 or 96 reactions
Storage-20°C-20°C-20°C
TransportPositive coldPositive coldDry ice
DestinationsInternationalInternationalFrance and Europe

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