SARS-CoV2 virus detection kits by real-time PCR

Technical :

These real-time PCR kits allow specific detection of SARS-CoV2 virus RNA. The PCR technique, which uses different fluorescent probes and specific primers, allows the amplification of specific genes found in the genome of the SARS-CoV2 RNA virus. The RNAs extracted from the different samples (nasopharyngeal swab, nasopharyngeal aspiration, expectoration, BAL, bronchial aspiration or saliva) are subjected to this test to make it possible to determine the persons carrying the SARS-CoV2 virus.

We also sell RNA extraction kits on the various samples recommended for the detection of the SARS-CoV2 virus (link).

Pre-analytical conditions :

Sample Type: Nasopharyngeal swab, nasopharyngeal aspiration, sputum, BAL, bronchial aspiration, saliva (only in symptomatic patients if nasopharyngeal cannot be performed)

Storage temperature during transport: 4 ° C

PRODUCTS :

  • SARS_CoV_2_one_step: Real-time PCR kit for the detection of SARS-CoV2, pathogen of the COVID-19 pandemic.
    • Ready-to-use kit: Two tubes of Master mix containing the RT and Taq enzymes , the primers and probes specific for each target and a positive control tube.
    • Targets: one sequence in the E gene and two distinct sequences in the Rdrp gene of the SARS-Cov2 virus and one sequence in the beta-actin gene serving as internal control of extraction and amplification.
    • Contains two duplex PCR:
      • PCR 1: Gene E in FAM and beta-actin in HEX
      • PCR 2: Rdrp1 in ROX and Rdrp2 in Cy5
    • Results in 2 h
    • Packaging: Available in tube of 96 reactions
    • Transport and storage: Delivery to France and Europe in dry ice and must be stored at – 20 ° C upon receipt
    • CE marked kit and validated by ANSM
  • SARS-CoV-2_E_RN: Real-time PCR kit for the detection of SARS-CoV2, pathogen of the COVID-19 pandemic.
    • Ready-to-use kit: One tube of Master mix containing Taq, primers and probes specific for each target, a tube containing RT and a positive control tube.
    • Targets: A sequence in the N gene, a sequence in the Rdrp gene of the SARS-Cov2 virus and a sequence in the beta-actin gene serving as internal control of extraction and amplification.
    • Contains a triplex PCR: N gene in ROX, Rdrp1 in Cy5 and beta-actin in HEX.
    • Results in 2 hours
    • Packaging: Available in tube of 96 reactions
    • Transport and storage: International delivery in positive cold and must be stored at -20 ° C upon receipt
    • CE marked kit and validated by ANSM
  • SARS-CoV-2_U_RN: Real-time PCR kit for the detection of SARS-CoV2, pathogen of the COVID-19 pandemic.
    • Ready-to-use kit: A tube of Master mix containing RT and Taq enzymes, primers and probes specific for each target and a positive control tube.
    • Targets: A sequence in the N gene, a sequence in the Rdrp gene of the SARS-Cov2 virus and a sequence in the beta-actin gene serving as an internal control of extraction and amplification.
    • Contains a triplex PCR: N gene in ROX, Rdrp1 in Cy5 and beta-actin in HEX.
    • Results in 2 hours
    • Packaging: Available in tube of 96 reactions
    • Transport and storage: Delivery to France and Europe in dry ice and must be stored at -20 ° C upon receipt
    • CE marked kit and validated by ANSM
ReferencesSARS_CoV_2_one_stepSARS-CoV-2_E_RNSARS-CoV-2_U_RN
Process PCR One stepOne stepOne step
Target genesE (FAM)
Rdrp1 (ROX)
Rdrp2 (Cy5)
N (FAM)
Rdrp (Cy5)  
N (FAM)
Rdrp (Cy5)
Internal controlBeta-actin (HEX)Beta-actin (HEX)Beta-actin (HEX)
Sensitivity
(copy/PCR)
E (20.1)
RdRp1 (20.1)
RdRp2 (20.1)
N (10)
RdRp (5)
N (20.1)
RdRp (5)
Nbr of wells211
ValidationANSMANSMANSM
CertificationCECECE
UseReady-to-useTo reconstituteReady-to-use
Packaging96 reactions96 reactions96 reactions
Storage-20°C-20°C-20°C
TransportDry icePositive coldDry ice
DestinationsFrance and EuropeInternationalFrance and Europe

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