PRRS diagnostic protocole including automated platform

PROTOCOLEAutomate metrologyTroubleshooting - Automate

Rev 1.57 / 2017-08-01

TARGET : PRRS North America / Chinese Strains


  • Tecan 8 channels + RoMA.
  • qPCR Agilent Mx3005

Purification system :  silica microbeads


Samples preparation - Semen

RNA Extraction - purification - Robotic method

qPCR plate preparation - Robotic method

Starting the QPCR program - template TaqMan

Results analysis using ADN_SOFT


In case of error, initialization error, please check that there was no collision done by a bad positioning of tube, rack, plates… As the robot initialize its position on each start, it is possible to clear problem by switching off the robot (wait 3 sec) and re switching on. Restart Gemini and initialization.
« Device is not implemented », happening during initialization Cause : Can be caused by a wrong initial position which causes an mechanical problem.   Corrective Task : Turn off Pipetting Robot, move the Robotic Arm on any another position of X, Y and rotation, then re launch Pipetting Robot and Gemini software.
Diluter error “tip is broken” Cause : This problem can occur when a wrong dispense operation has been done. The robot or the operator “Desactivated “ the tip. Corrective Task :  To repair this, Setup -> Configuration -> LiHa -> correct the check box where the tip is marked as broken.
Diluter error “Plunger overload” The robot stops until operator click on “initialize”. Once re initialized (click on Initialize), the robot can continue the run. If the robot doesn’t succeed in initialization procedure, switch off the robot, check if needle is not clogged, move diluter (the screw under syringe) up and down and retest. Cause :  This error can mean that needle is blocked or diluter is damaged.   Corrective Task : If the problem occurs more than 1 time each 5 runs on the same channel (which would be not plugged), diluter has to be changed. Procedure is as follows : 0- Switch off the robot, set the robotics arms in the middle in order to be able to lift the hood. 1- Unscrew bottom syringe screw 2- Put down the arm 3- Unscrew the syringe, set it on the other new diluter 4- Unscrew the 2 top tubes – BE CAREFULL OF WATER WHICH COULD DROP DOWN FROM THE 2 TUBES TO ELECTRONICS COMPONENTS OF THE ROBOT/DILUTER (you can put tubes on a wipe to absorb water.) 5- Check below the diluter if any screw retains diluter, and if there is one, unscrew it too. 6- Remove the old diluter 7- Check the address behind it, and report the same on the new one (1st diluter is “0”, 2nd is “1”, etc.) 8- Set the new one, - BE CAREFULL OF WATER WHICH COULD DROP DOWN FROM THE 2 TUBES TO ELECTRONICS COMPONENTS OF THE ROBOT/DILUTER 9- Screw firmly tubes, then syringe, then arm syringe. 10- Start robot, start Gemini and initialize. If error occurs, recheck address increment is correctly set.  
Detection error “no liquid”   Cause :   can be caused by : -  Not enough liquid in the tube/troughs -  Deionized liquid in the tube/trough so the robot cannot detect it. -  ADN_SOFT underestimated the needed volume (in this case, please contact ), -  Evaporation of the liquid because too much time occurred between filling and program run.   Corrective Task Please refill the liquid, and click on “Retry Detection”. NB : If the volume inside looks “OK” for the estimated needed aspiration but the volume is so low that the robot can’t detect it, you can click on “Move tips to Zmax”, the robot will aspirate to the bottom of the tube / trough.