For more than 15 years, AD Nucléis has brought together experts in molecular biology and robotics from different backgrounds: production technicians, engineers, doctors. This combination of skills provides a unique know-how allowing the architecture of any diagnosis for your laboratory: organisation of control plans, sample preparation methods, quantitative PCR, process robotisation, quality issues…
AD Nucléis is a French designer and manufacturer of real-time PCR reagents and robotics. It is ISO 13485 certified by AB Certification and is supported by the Auvergne Rhône-Alpes Region, BPIFrance and the French Ministry of Economic Recovery.
AD Nucléis’ training courses cover all aspects of PCR analysis and robotics: sample preparation, nucleic acid extraction and purification, real-time PCR, quality and good laboratory practice.
Quantitative PCR
PCR: General principle
- Bons usages et pratique de la PCR
- Importance de la matrice sur la recherche de maladie
- Le prélèvement et son contenant
- Maladie et méthode de référence
Training: Development of quantitative PCR test kits
- Principe général
- Les composants d’un mix de PCR
- Design des amorces et cible
- Cycle thermique et optimisation de la température
- Technologies de fluorescence
- Optimisation d’un kit de détection
- Validations analytiques et cliniques
- Analyse de courbes de PCR Validation d’un kit PCR : sensibilité, spécificité, linéarité, robustesse, stabilité
- Aspect statistique : test de probit et cie
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